Coding

Part:BBa_K3739001

Designed by: Han Ziyan   Group: iGEM21_XMU-China   (2021-09-08)


his-CBM-GFP

CBM can anchor onto cellulose and GFP can show whether CenA anchor or not becteria that is used to store our plasmid


Usage and Biology

CBM

Cellulose enzymes have two domains, and the one that helps bind to cellulose is called cellulose binding module (CBM), and therefore it helps our fusion protein bind to cellulose-rich cell wall. Here we choose the CBM of CenA from Cellulomonas fimi, which has been successfully expressed in Escherichia coli.

GFP

Protein tags are peptides genetically fused to the proteins of interest. There are many different kinds of tags developed for different purposes. For labeling experiments, normally the proteins of interest are tagged directly by fluorescent tags. Green fluorescent protein (GFP) make it easier to study protein localization, interactions and dynamics when fusing with other peptides and proteins.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 286
    Illegal AgeI site found at 376
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 439


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Categories
Parameters
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