Coding
Part:BBa_K3738022:Experience
Designed by: Emily Hagens and Mark Lea Group: iGEM21_Lethbridge (2021-10-19)
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Applications of BBa_K3738022
Our two RNA constructs are used to express the guide-RNAs that would have been used with our CRISPR system. For these constructs, we used in-vitro transcription to express the RNA segments of interests, and used urea PAGEs to confirm successful transcription. Both of these were successful, and did not require troubleshooting in the ways that the DNA constructs did.
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