Part:BBa_K3738019:Design
MlrA for E.coli
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 7
Illegal EcoRI site found at 868 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 7
Illegal EcoRI site found at 868 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 7
Illegal EcoRI site found at 868 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 7
Illegal EcoRI site found at 868 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 7
Illegal EcoRI site found at 868 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
Codon optimized for E.coli.
Source
Sphingomonas sp. USTB-05
References
Saito T, Okano K, Park H-D, Itayama T, Inamori Y, Neilan BA, et al. Detection and sequencing of the microcystin LR-degrading gene, mlrA, from new bacteria isolated from Japanese lakes. FEMS Microbiology Letters. 2003;229(2):271-6. doi: 10.1016/S0378-1097(03)00847-4.
Jiang Y, Shao J, Wu X, Xu Y, Li R. Active and silent members in the mlr gene cluster of a microcystin-degrading bacterium isolated from Lake Taihu, China. FEMS Microbiology Letters. 2011;322(2):108-14. doi: 10.1111/j.1574-6968.2011.02337.x.
Wei J, Huang F, Feng H, Massey IY, Clara T, Long D, et al. Characterization and Mechanism of Linearized-Microcystinase Involved in Bacterial Degradation of Microcystins. Frontiers in Microbiology. 2021;12(527). doi: 10.3389/fmicb.2021.646084.