Composite

Part:BBa_K3738018:Design

Designed by: Emily Hagens and Mark Lea   Group: iGEM21_Lethbridge   (2021-10-17)


MS2 Bacteriophage Capsid Protein Dimer (Composite Part)


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 183
    Illegal PstI site found at 591
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 183
    Illegal PstI site found at 591
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 183
    Illegal PstI site found at 591
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 183
    Illegal PstI site found at 591
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The MS2 capsid protein coding region in this part has been codon-optimized for E.coli.


Source

MS2 Bacteriophage

References

de Martín Garrido, N., Crone, M.A., Ramlaul, K., Simpson, P.A., Freemont, P.S. and Aylett, C.H.S. (2020), Bacteriophage MS2 displays unreported capsid variability assembling T = 4 and mixed capsids. Mol Microbiol, 113: 143-152. https://doi.org/10.1111/mmi.14406