Composite

Part:BBa_K3738018

Designed by: Emily Hagens and Mark Lea   Group: iGEM21_Lethbridge   (2021-10-17)


MS2 Bacteriophage Capsid Protein Dimer (Composite Part)

The MS2 capsid protein comprises most of the capsid of the MS2 phage, as well as MS2 phage-like particles (PLPs). A single MS2 capsid requires 178 copies of the capsid protein, as one copy of maturation protein A (BBa_K3738000), which is critical in allowing the capsid to bind to the cell and deliver its cargo. This composite part includes a coding region comprised of 2 copies of the MS2 capsid protein, joined by a histidine tag flanked by 2 linker sequences (BBa_K3738008). It also contains the IPTG-inducible T7 promoter (BBa_J64997), RBS BBa_B0034, and double terminator BBa_B0015.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 183
    Illegal PstI site found at 591
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 183
    Illegal PstI site found at 591
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 183
    Illegal PstI site found at 591
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 183
    Illegal PstI site found at 591
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None