Generator

Part:BBa_K3737001:Design

Designed by: Matthew Hanson   Group: iGEM21_Alma   (2021-10-19)


Estrogen receptor driven by the T7 Promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 908
    Illegal BamHI site found at 570
    Illegal XhoI site found at 464
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

used the T7 promoter because the estrogen receptor may have been toxic.


Source

DNA synthesis from the company IDT

References