Coding

Part:BBa_K3735002:Design

Designed by: Jiandong Jiang   Group: iGEM21_NAU-CHINA   (2021-10-01)


Coding sequence


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 135
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

aa


Source

Gene ssuE comes from the ssuEADCB gene cluster, which is required for the utilization of alkanesulfonates as sulfur sources, and is expressed under conditions of sulfate or cysteine starvation. Escherichia coli BL21 also has this gene cluster. The gene sequence of this part was obtained from NCBI and its GenBank Accession number is: 8182327.

References