Composite

Part:BBa_K3733046:Design

Designed by: Zhenhao Han   Group: iGEM21_HZAU-China   (2021-10-01)


RNA Thermometer(heat-inducible) test system


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 158
    Illegal AgeI site found at 281
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This is a composite part including the RNA thermometer(hot-activate) and the neGFP fluorescent protein.


Source

Used a strong RBS to produce high levels of expression of the neGFP fluorescent protein.

References