Part:BBa_K3733045
RNA Thermometer(heat-repressible) test system
Use a constitutive promoter (BBa_J23110), RNA thermometer (BBa_K3733011), strong RBS (BBa_B0034), fluorescent protein neGFP (BBa_K3733012), and an artificial terminator (BBa_B1006). Will express high levels of fluorescent protein below 28 ℃.
Usage and Biology
This is a composite part constructed to verify the effectiveness of the heat-repressible RNA thermometer.
Functional Parameters
We transformed this composite part into E.coli DH5α. We cultured both experimental group and control group at both 37 ℃ and 28 ℃ for 12 hours and detected RFU / OD600 by a microplate reader. As the result shown in Figure 1, RFU / OD600 data of the media at 28 ℃ are higher than ones at 37 ℃, which could explain the RNA thermometer is valid.
Sequence and Features
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 149
Illegal AgeI site found at 272 - 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 82
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