Part:BBa_K3733036:Experience

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Applications of BBa_K3733036

We transform E.coli DH5α strain with BBa_K3733036 and chose neGFP(https://parts.igem.org/Part:BBa_K3733012) as the report. The strain was expanded in LB medium to OD=0.4, then 198μL of bacterial solution was injected into a 96-well plate, and a series of concentration gradients (0mM, 0.0001mM, 0.001mM, 0.01mM, 0.1mM, 1mM) of sodium thiosulfate were added into each well to 200μL. Measure the fluorescence with gain of 75 and OD₆₀₀ in the Synergy H1 microplate reader overnight. The results of our experiment are shown in the figure below (Figure 1).

Figure 1.The curve of OD₆₀₀ corrected RFU values change with time under induction of different concentrations of thiosulfate

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