Composite

Part:BBa_K3733034

Designed by: Zhenhao Han   Group: iGEM21_HZAU-China   (2021-10-01)


The thiosulfate sensing system under the control of J23105

This part could sense different concentrations of thiosulfate and reflects this difference in fluorescence intensity. Its functionality depends on a two-component system ThsSR, which each constitute a sensor histidine kinase and a response regulator. The ThsS is sensor histidine kinase, which can phosphorylate ThsR in response to thiosulfate. And then the phosphorylated ThsR will binding the site and activate the transcription of PphsA promoter. In this system, we use J23105 to control the expression of ThsS and ThsR, which in turn controls the differentiation of the system to different thiosulfate concentrations.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NheI site found at 1151
    Illegal NheI site found at 1867
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 550
    Illegal BamHI site found at 598
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 3065
    Illegal AgeI site found at 3188
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 1083
    Illegal SapI.rc site found at 1909


[edit]
Categories
Parameters
None