Composite
Part:BBa_K3733034
Designed by: Zhenhao Han Group: iGEM21_HZAU-China (2021-10-01)
The thiosulfate sensing system under the control of J23105
This part could sense different concentrations of thiosulfate and reflects this difference in fluorescence intensity. Its functionality depends on a two-component system ThsSR, which each constitute a sensor histidine kinase and a response regulator. The ThsS is sensor histidine kinase, which can phosphorylate ThsR in response to thiosulfate. And then the phosphorylated ThsR will binding the site and activate the transcription of PphsA promoter. In this system, we use J23105 to control the expression of ThsS and ThsR, which in turn controls the differentiation of the system to different thiosulfate concentrations.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30
Illegal NheI site found at 1151
Illegal NheI site found at 1867 - 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 550
Illegal BamHI site found at 598 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 3065
Illegal AgeI site found at 3188 - 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 1083
Illegal SapI.rc site found at 1909
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Categories
Parameters
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