Composite
Part:BBa_K3733027:Design
Designed by: Yuanhui Sun Group: iGEM21_HZAU-China (2021-10-01)
The nitrate sensor(J23115-J61100) system with EBS-PyeaR and neGFP
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 337
Illegal BglII site found at 505 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 1089
Illegal AgeI site found at 1212 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
The NarX and NarL gene are overlapping genes in E.coli MG1655 genome. If you want to use NarX and NarL protein separately, please to notice the overlap.
Source
The NarXL two-component signal system and PyeaR promoter come from E.coli MG1655 genome. The extra NarL binding site comes from PyeaR.