Composite

Part:BBa_K3733015:Design

Designed by: Yuanhui Sun   Group: iGEM21_HZAU-China   (2021-10-01)


The nitrate sensor(J23100-B0034) system with neGFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2018
    Illegal BglII site found at 2186
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 2770
    Illegal AgeI site found at 2893
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The NarX and NarL gene are overlapping genes in E.coli MG1655 genome. If you want to use NarX and NarL protein separately, please to notice the overlap.


Source

The NarXL two-component signal system and PyeaR promoter come from E.coli MG1655 genome.

References