Coding
Part:BBa_K3733006:Design
Designed by: Zhenhao Han Group: iGEM21_HZAU-China (2021-09-28)
ScGS: Streptomyces coelicolor geosmin synthase
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Codon optimization has been applied on the 2181 bp sequence from Streptomyces coelicolor A3(2) to improve gene expression.
Source
The original sequence SCO6073 comes from Genbank(NC_003888).
References
Harris G G, Lombardi P M, Pemberton T A, et al. Structural Studies of Geosmin Synthase, a Bifunctional Sesquiterpene Synthase with αα Domain Architecture That Catalyzes a Unique Cyclization–Fragmentation Reaction Sequence[J]. Biochemistry, 2015, 54(48): 7142-7155.