Regulatory

Part:BBa_K3733005:Design

Designed by: Zhenhao Han   Group: iGEM21_HZAU-China   (2021-09-28)


J23106a:Improved promoter of J23106


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We replaced the -35 region sequence of the J23106 promoter with nucleotides 3-5 from TAC to ATA, but did not change the -10 region.


Source

The part is improved from BBa_J23106.

References