Coding

Part:BBa_K3733003:Design

Designed by: Zhenhao Han   Group: iGEM21_HZAU-China   (2021-09-28)


ThsS(L547T)：A point mutation of ThsS


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We optimized the codon of ThsS based on Escherichia coli codon bias, and mutated the amino acid at position 547 of ThsS from leucine to threonine.


Source

The part is improved from ThsS(BBa_K2507000).

References

Landry B P, Palanki R, Dyulgyarov N, et al. Phosphatase activity tunes two-component system sensor detection threshold[J]. Nature communications, 2018, 9(1): 1-10.