Part:BBa_K3731002:Design
vgb
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
PCR-amplified CFppk1-vgb-mazE was digested with restriction enzymes Kpn I and Hind III, after which it was insert into corresponding multiple cloning sties of the broad-host-range expressing vector pBBR1MCS2.
Source
E.Coli BL21 was purchased from China Center of Industrial Culture Collection (CICC, China). For the construction of pBBR1MCS2-ppk1-vgb-mazE, genomic DNA of E.Coli BL21 was used as the template to PCR-amplify ppk1, vgb and mazE with primers.
References
Kallio, P. T.; Kim, D.; Tsai, P. S.; Bailey, J. E. Intracellular expression of Vitreoscilla hemoglobin alters Escherichia coli energy metabolism under oxygen-limited conditions. The FEBS Journal 1994, 219 (1-2), 201-208.
Kanak L. Dikshit, Rajendra P. Dikshit, Dale A. Webster, Study of Vitreoscilla globin(vgb) gene expression and promoter activity in E. Coli through transcriptional fusion , Nucleic Acids Research, Volume 18, Issue 14, 25 July 1990, Pages 4149–4155.