Part:BBa_K3726121
L2_BOH1ABC-FRRRF
This is a composite part that corresponds with a level 2 MoClo part. It has three transcriptional units for the expression of the Lv1 parts âBBa_K3726044â BOH1_A_LV1, âBBa_K3726045â BOH1_B_LV1 and âBBa_K3726051â BOH1_C_FRRRF_LV1.
This level 2 part has as a function expressing the necessary enzymes for butanol biosynthesis through an artificial pathway ( X. Liu, R. Miao, P. Lindberg y P. Lindblad, âModular engineering for efficient photosynthetic biosynthesis of 1-butanol from CO2 in cyanobacteria").
This part has been assembled following the marburg collection standard, then the transcriptional units are flanked by two homology regions âBBa_K3726104â 5CON1(H)_NS1(mod)-up (PCC 11801) and âBBa_K3726105 â 3CON5(H)_NS1(mod)-down (PCC 11801) that allows the double homologous recombination within the genome of PCC 11801.
Sequence and Features
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 1230
Illegal EcoRI site found at 1850
Illegal PstI site found at 18
Illegal PstI site found at 1755
Illegal PstI site found at 2003
Illegal PstI site found at 3988
Illegal PstI site found at 4237
Illegal PstI site found at 7860 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 1230
Illegal EcoRI site found at 1850
Illegal NheI site found at 6540
Illegal NheI site found at 6563
Illegal NheI site found at 6866
Illegal NheI site found at 7094
Illegal PstI site found at 18
Illegal PstI site found at 1755
Illegal PstI site found at 2003
Illegal PstI site found at 3988
Illegal PstI site found at 4237
Illegal PstI site found at 7860 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 1230
Illegal EcoRI site found at 1850
Illegal BglII site found at 8255
Illegal BglII site found at 8372
Illegal BglII site found at 10791
Illegal BamHI site found at 4320
Illegal BamHI site found at 4659
Illegal XhoI site found at 5568
Illegal XhoI site found at 9475
Illegal XhoI site found at 10318 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 1230
Illegal EcoRI site found at 1850
Illegal PstI site found at 18
Illegal PstI site found at 1755
Illegal PstI site found at 2003
Illegal PstI site found at 3988
Illegal PstI site found at 4237
Illegal PstI site found at 7860 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 1230
Illegal EcoRI site found at 1850
Illegal PstI site found at 18
Illegal PstI site found at 1755
Illegal PstI site found at 2003
Illegal PstI site found at 3988
Illegal PstI site found at 4237
Illegal PstI site found at 7860
Illegal NgoMIV site found at 1494
Illegal NgoMIV site found at 1594
Illegal NgoMIV site found at 1900
Illegal NgoMIV site found at 4915
Illegal AgeI site found at 594
Illegal AgeI site found at 2272
Illegal AgeI site found at 3443
Illegal AgeI site found at 4443 - 1000COMPATIBLE WITH RFC[1000]
None |