Composite

Part:BBa_K3726121

Designed by: Santiago Barrag�n Ariza   Group: iGEM21_MADRID_UCM   (2021-10-20)


L2_BOH1ABC-FRRRF

This is a composite part that corresponds with a level 2 MoClo part. It has three transcriptional units for the expression of the Lv1 parts “BBa_K3726044” BOH1_A_LV1, “BBa_K3726045” BOH1_B_LV1 and “BBa_K3726051” BOH1_C_FRRRF_LV1.

This level 2 part has as a function expressing the necessary enzymes for butanol biosynthesis through an artificial pathway ( X. Liu, R. Miao, P. Lindberg y P. Lindblad, “Modular engineering for efficient photosynthetic biosynthesis of 1-butanol from CO2 in cyanobacteria").

This part has been assembled following the marburg collection standard, then the transcriptional units are flanked by two homology regions “BBa_K3726104” 5CON1(H)_NS1(mod)-up (PCC 11801) and “BBa_K3726105 ” 3CON5(H)_NS1(mod)-down (PCC 11801) that allows the double homologous recombination within the genome of PCC 11801.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1230
    Illegal EcoRI site found at 1850
    Illegal PstI site found at 18
    Illegal PstI site found at 1755
    Illegal PstI site found at 2003
    Illegal PstI site found at 3988
    Illegal PstI site found at 4237
    Illegal PstI site found at 7860
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1230
    Illegal EcoRI site found at 1850
    Illegal NheI site found at 6540
    Illegal NheI site found at 6563
    Illegal NheI site found at 6866
    Illegal NheI site found at 7094
    Illegal PstI site found at 18
    Illegal PstI site found at 1755
    Illegal PstI site found at 2003
    Illegal PstI site found at 3988
    Illegal PstI site found at 4237
    Illegal PstI site found at 7860
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1230
    Illegal EcoRI site found at 1850
    Illegal BglII site found at 8255
    Illegal BglII site found at 8372
    Illegal BglII site found at 10791
    Illegal BamHI site found at 4320
    Illegal BamHI site found at 4659
    Illegal XhoI site found at 5568
    Illegal XhoI site found at 9475
    Illegal XhoI site found at 10318
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1230
    Illegal EcoRI site found at 1850
    Illegal PstI site found at 18
    Illegal PstI site found at 1755
    Illegal PstI site found at 2003
    Illegal PstI site found at 3988
    Illegal PstI site found at 4237
    Illegal PstI site found at 7860
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1230
    Illegal EcoRI site found at 1850
    Illegal PstI site found at 18
    Illegal PstI site found at 1755
    Illegal PstI site found at 2003
    Illegal PstI site found at 3988
    Illegal PstI site found at 4237
    Illegal PstI site found at 7860
    Illegal NgoMIV site found at 1494
    Illegal NgoMIV site found at 1594
    Illegal NgoMIV site found at 1900
    Illegal NgoMIV site found at 4915
    Illegal AgeI site found at 594
    Illegal AgeI site found at 2272
    Illegal AgeI site found at 3443
    Illegal AgeI site found at 4443
  • 1000
    COMPATIBLE WITH RFC[1000]


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