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Part:BBa_K3726105:Design

Designed by: Lucia Tapia   Group: iGEM21_MADRID_UCM   (2021-10-08)


3CON5(H)_NS1(mod)-down (PCC 11801)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 339
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 34


Design Notes

The part is a marburg collection design MoClo Lv.0 part, assembled in the universal phytobrick entry vector BBa_K2560001. As a 5’CON part the upstream overhang is CGCT, while the downstream overhang is AGCT.

The Sapl site should be removed in order to make this part RFC1000 compatible. however, this part is MoClo compatible with the marburg collection.

Source

Homology connector has been found within the genome of Synechococcus elongatus PCC 11801.

Genome available at https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?id=2219813