RBS

Part:BBa_K3726086:Design

Designed by: Lucia Tapia   Group: iGEM21_MADRID_UCM   (2021-10-08)


RBS BCD2(Mod)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The part has been assembled in accordance with the design guidelines of Marburg Collection https://2018.igem.org/Team:Marburg/Part_Collection. Despite that the part sequence does not include the resulting MoClo Overhangs after level 1 assembly. Then the level 0 part includes the overhangs TACTAGAG in the 5’ end of the sequence, and the bases TAATCAATG in the 3’ end of the sequence.

Source

To know more about Marburg collection guidelines visit https://2018.igem.org/Team:Marburg/Part_Collection

References

[4]V. Mutalik, J. Guimaraes, G. Cambray, C. Lam, M. Christoffersen, Q. Mai, A. Tran, M. Paull, J. Keasling, A. Arkin and D. Endy, "Precise and reliable gene expression via standard transcription and translation initiation elements", Nature Methods, vol. 10, no. 4, pp. 354-360, 2013.