Regulatory

Part:BBa_K3726081:Design

Designed by: Santiago Barragán Ariza   Group: iGEM21_MADRID_UCM   (2021-10-08)


P_Ptrc20


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The part is a phytobrick compatible MoClo Lv.0 part, assembled in the universal phytobrick entry vector BBa_K2560001. As a promoter part, the upstream overhang is GGAG, while the downstream overhang is TACTAGAG , where AGAG are extra bases for optimal ribosomal binding, in accordance with the Marburg Collection design guidelines.

Source

Ptrc2O is a modified version of Ptrc1O which is a hybrid between Escherichia coli trp promoter and lacUV5 promoter.

References

H. Huang, D. Camsund, P. Lindblad and T. Heidorn, "Design and characterization of molecular tools for a Synthetic Biology approach towards developing cyanobacterial biotechnology", 2021.