Regulatory

Part:BBa_K3726078

Designed by: Santiago Barragán Ariza   Group: iGEM21_MADRID_UCM   (2021-10-08)


P_Pgntk

This part is a phytobrick compatible MoClo Lv.0 promoter part adaptation of BBa_K2194002. The promoter has been assembled in the universal phytobrick entry vector BBa_K2560001.

pgntk is a negative feedback membrane stress promoter from E. Coli that has been shown to optimize expression levels of Acrb efflux pumps in order to cope with membrane-related stress.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None