Regulatory

Part:BBa_K3726076

Designed by: Santiago Barragán Ariza   Group: iGEM21_MADRID_UCM   (2021-10-08)


P_pBAD

This part is a device for arabinose-regulated expression of genes. The part is a phytobrick compatible MoClo Lv.0 promoter part adaptation of BBa_I0500, assembled in the universal phytobrick entry vector BBa_K2560001. The part is comprised by an AraC regulator expression cassette, followed by a BBa_I13453 derived pBAD promoter sequence.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1205
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1144
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 979
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 961


[edit]
Categories
Parameters
None