Composite

Part:BBa_K3726073

Designed by: Santiago Barrag�n Ariza   Group: iGEM21_MADRID_UCM   (2021-10-19)


L2_TOL_ABB

This is a composite part that corresponds with a level 2 MoClo part. It has three transcriptional units for the expression of the Lv1 parts “BBa_K3726066” Lv1_ HspA-A, “BBa_K3726071” Lv1_AcrbV2 - B and “BBa_K3726069” Lv1 SigB-B.

This level 2 integrative part has as a function expressing all “tolerance and secretion” constructs within PCC 11801 Genome. It is a system that combines the simultaneous effect of all the parts, to study their influence when combining the effects and separately.

This part has been assembled following the marburg collection standard, then the transcriptional units are flanked by two homology regions “BBa_K3726108” 5CON1(H)_NS3(mod)-up (PCC 11801) and “BBa_K3726109” 3CON5(H)_NS3(mod)-down (PCC 11801) that allows the double homologous recombination within the genome of PCC 11801.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1139
    Illegal EcoRI site found at 7738
    Illegal PstI site found at 798
    Illegal PstI site found at 3917
    Illegal PstI site found at 8640
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1139
    Illegal EcoRI site found at 7738
    Illegal NheI site found at 3646
    Illegal NheI site found at 7319
    Illegal NheI site found at 7342
    Illegal NheI site found at 8365
    Illegal PstI site found at 798
    Illegal PstI site found at 3917
    Illegal PstI site found at 8640
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1139
    Illegal EcoRI site found at 7738
    Illegal XhoI site found at 2860
    Illegal XhoI site found at 7774
    Illegal XhoI site found at 8011
    Illegal XhoI site found at 8793
    Illegal XhoI site found at 8913
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1139
    Illegal EcoRI site found at 7738
    Illegal PstI site found at 798
    Illegal PstI site found at 3917
    Illegal PstI site found at 8640
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1139
    Illegal EcoRI site found at 7738
    Illegal PstI site found at 798
    Illegal PstI site found at 3917
    Illegal PstI site found at 8640
    Illegal NgoMIV site found at 724
    Illegal NgoMIV site found at 5053
    Illegal NgoMIV site found at 6569
    Illegal AgeI site found at 3003
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 7498


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