Part:BBa_K3726070
Lv.1 AcrbV2 - A
This is a composite part that corresponds with a level 1 MoClo part in accordance with the marburg collection standard. It is a transcriptional unit for the expression of the part “BBa_K3726065” L0_AcrbV2 which codifies a membrane transporter subunit , which has been optimized for the specific secretion of n-butanol. Within our project we have used AcrbV2 to enhance n-butanol secretion through cell membrane in order to cut down on toxicity generated by itself.
The part is flanked by two connectors that will allow the assembly of a Lv2 construct. In its upstream region the connector is 5'Con2 Bba_K2560066, and in its downstream region 3'Con2 Bba_K2560071 .
Expression is controlled by the Medium-Strong inducible promoter pBAD “BBa_K3726076” which is regulated by the presence of Arabinose, and the strong synthetic RBS, RBS_PtaBs “BBa_K3726092”
Sequence and Features
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 2033
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1634
Illegal NheI site found at 1762
Illegal PstI site found at 2033 - 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 1573
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 2033
- 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 2033
Illegal NgoMIV site found at 3169
Illegal NgoMIV site found at 4685
Illegal AgeI site found at 109
Illegal AgeI site found at 1408 - 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 1390
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