Part:BBa_K3726068

Lv.1 SigB - A

This is a composite part that corresponds with a level 1 MoClo part in accordance with the marburg collection standard. It is a transcriptional unit for the expression of the part “BBa_K3726063” L0_SigB which codifies a sigma factor that when it is over-expressed increases tolerance to temperature, and reduces ROS stress. Within our project we have used SigB to reduce ROS stress generated from n-butanol.

The part in its upstream region, has the connector 5'Con3 “K2560067” that will allow the assembly of a Lv2 construct following the marburg collection standard. In its downstream region has a homology region “BBa_K3726109” 3CON5(H)_NS3(mod)-down (PCC 11801) for homologous recombination within the genome of PCC 11801.

Expression is controlled by the strong constitutive promoter Prbcl-m36 “BBa_K3726080” and the strong synthetic RBS, RBS_PtaBs “BBa_K3726092”.

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal EcoRI site found at 1103
  Illegal PstI site found at 649
  Illegal PstI site found at 2005
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal EcoRI site found at 1103
  Illegal NheI site found at 1730
  Illegal PstI site found at 649
  Illegal PstI site found at 2005
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal EcoRI site found at 1103
  Illegal XhoI site found at 1139
  Illegal XhoI site found at 1376
  Illegal XhoI site found at 2158
  Illegal XhoI site found at 2278
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal EcoRI site found at 1103
  Illegal PstI site found at 649
  Illegal PstI site found at 2005
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal EcoRI site found at 1103
  Illegal PstI site found at 649
  Illegal PstI site found at 2005
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal SapI.rc site found at 863