Part:BBa_K3726066:Design
Lv.1 HspA - A
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 1139
Illegal PstI site found at 798 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 1139
Illegal PstI site found at 798 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 1139
Illegal XhoI site found at 2860 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 1139
Illegal PstI site found at 798 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 1139
Illegal PstI site found at 798
Illegal NgoMIV site found at 724 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
The part is a MoClo Lv.1 part, assembled in the acceptor Lv1 entry vector “BBa_K3228069” P1_pANs_SpecR, which is a self replicating shuttle vector. This part has been assembled following the marburg collection standard, then the transcriptional unit is flanked by the part“BBa_K3726108” 5CON1(H)_NS3(mod)-up (PCC 11801) that allows homologous recombination within the PCC 11801 Genome, and by the part 3'Con1 “BBa_K2560070” that will allow the assembly of a Lv2 construct.
This design will allow us to test which “tolerance and secretion ” variant has better efficiency by its own account as a replicative vector, and then assemble them as an integrative Lv2 Moclo construct. To know more about this, visit our webpage: https://2021.igem.org/Team:MADRID_UCM/Design
Source
This construct has been made by golden gate reaction.
References
"Using Transcriptomics To Improve Butanol Tolerance of Synechocystis sp. Strain PCC 6803 | Applied and Environmental Microbiology", Applied and Environmental Microbiology, 2021.