Part:BBa_K3726066
Lv.1 HspA - A
This is a composite part that corresponds with a level 1 MoClo part in accordance with the marburg collection standard. It is a transcriptional unit for the expression of the part âBBa_K3726061â L0_HspA* which codifies the major heat shock protein capable of enhancing cellular fitness in the presence of environmental stresses such as solvent presence.
Within our project we have used HspA to reduce ROS stress generated from n-butanol.
The part in its upstream region has a homology region âBBa_K3726108â 5CON1(H)_NS3(mod)-up (PCC 11801) for homologous recombination within the genome of PCC 11801. In the downstream region, the part has the connector 3'Con1 âBBa_K2560070â that will allow the assembly of a Lv2 construct following the marburg collection standard.
Likewise, a chloramphenicol resistance cassette is included downstream the transcriptional unit region.
Expression is controlled by the strong constitutive promoter P_PpsbA*-RiboJ âBBa_K3726079â and the strong synthetic RBS, RBS_PtaBs âBBa_K3726092â
Sequence and Features
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 1139
Illegal PstI site found at 798 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 1139
Illegal PstI site found at 798 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 1139
Illegal XhoI site found at 2860 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 1139
Illegal PstI site found at 798 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 1139
Illegal PstI site found at 798
Illegal NgoMIV site found at 724 - 1000COMPATIBLE WITH RFC[1000]
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