Part:BBa_K3726062:Design

CDS_HspA(7942)

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

In the upstream region the bases taatc corresponds with 5bp a spacer sequence to improve ribosomal translation, while AATG overhang creates an start codon that forces translation initiation of the downstream coding sequence.

In the downstream region, the extra “aa” bases allow the creation of an additional stop codon (TAA) at the end of the upstream coding sequence, ending the translation of the desired CDS in accordance with the Marburg Collection design guidelines.

Source

Coding sequence of this HspA has been obtained from Synechococcus elongatus PCC 7942 genome through PCR. This sequence is codified in the gen SYNPCC7942_RS12200 of Synechococcus elongatus PCC 7942.

References

"Using Transcriptomics To Improve Butanol Tolerance of Synechocystis sp. Strain PCC 6803 | Applied and Environmental Microbiology", Applied and Environmental Microbiology, 2021. [Online]. Available: https://journals.asm.org/doi/10.1128/aem.02694-13?permanently=true.