Composite

Part:BBa_K3726059

Designed by: Santiago Barrag�n Ariza   Group: iGEM21_MADRID_UCM   (2021-10-20)


L2_BOH2A-FRRRF

This is a composite part that corresponds with a level 2 MoClo part. It has two transcriptional units for the expression of the Lv1 parts “BBa_K3726047” BOH2_A_LV1 and “BBa_K3726051” BOH1_C_FRRRF_LV1. This level 2 part has as a function expressing the necessary enzymes for butanol biosynthesis through an artificial pathway.

This part has been assembled following the marburg collection standard, then the transcriptional units are flanked by two homology regions “BBa_K3726104” 5CON1(H)_NS1(mod)-up (PCC 11801) and “BBa_K3726105 ” 3CON5(H)_NS1(mod)-down (PCC 11801) that allows the double homologous recombination within the genome of PCC 11801.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1230
    Illegal EcoRI site found at 1873
    Illegal PstI site found at 18
    Illegal PstI site found at 1778
    Illegal PstI site found at 2026
    Illegal PstI site found at 3327
    Illegal PstI site found at 6974
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1230
    Illegal EcoRI site found at 1873
    Illegal NheI site found at 5654
    Illegal NheI site found at 5677
    Illegal NheI site found at 5980
    Illegal NheI site found at 6208
    Illegal PstI site found at 18
    Illegal PstI site found at 1778
    Illegal PstI site found at 2026
    Illegal PstI site found at 3327
    Illegal PstI site found at 6974
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1230
    Illegal EcoRI site found at 1873
    Illegal BglII site found at 7369
    Illegal BglII site found at 7486
    Illegal BglII site found at 9905
    Illegal BamHI site found at 3410
    Illegal BamHI site found at 3749
    Illegal XhoI site found at 4682
    Illegal XhoI site found at 8589
    Illegal XhoI site found at 9432
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1230
    Illegal EcoRI site found at 1873
    Illegal PstI site found at 18
    Illegal PstI site found at 1778
    Illegal PstI site found at 2026
    Illegal PstI site found at 3327
    Illegal PstI site found at 6974
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1230
    Illegal EcoRI site found at 1873
    Illegal PstI site found at 18
    Illegal PstI site found at 1778
    Illegal PstI site found at 2026
    Illegal PstI site found at 3327
    Illegal PstI site found at 6974
    Illegal NgoMIV site found at 1517
    Illegal NgoMIV site found at 1617
    Illegal NgoMIV site found at 1923
    Illegal NgoMIV site found at 4005
    Illegal AgeI site found at 594
    Illegal AgeI site found at 2295
    Illegal AgeI site found at 3533
  • 1000
    COMPATIBLE WITH RFC[1000]


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