Composite

Part:BBa_K3726056:Design

Designed by: Santiago Barrag�n Ariza   Group: iGEM21_MADRID_UCM   (2021-10-19)


Lv.1_PKMCG-B


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 2173
    Illegal EcoRI site found at 2614
    Illegal EcoRI site found at 4373
    Illegal EcoRI site found at 5489
    Illegal EcoRI site found at 5573
    Illegal EcoRI site found at 8628
    Illegal PstI site found at 2758
    Illegal PstI site found at 2857
    Illegal PstI site found at 3442
    Illegal PstI site found at 4400
    Illegal PstI site found at 4628
    Illegal PstI site found at 6138
    Illegal PstI site found at 7152
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 2173
    Illegal EcoRI site found at 2614
    Illegal EcoRI site found at 4373
    Illegal EcoRI site found at 5489
    Illegal EcoRI site found at 5573
    Illegal EcoRI site found at 8628
    Illegal NheI site found at 5712
    Illegal PstI site found at 2758
    Illegal PstI site found at 2857
    Illegal PstI site found at 3442
    Illegal PstI site found at 4400
    Illegal PstI site found at 4628
    Illegal PstI site found at 6138
    Illegal PstI site found at 7152
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 2173
    Illegal EcoRI site found at 2614
    Illegal EcoRI site found at 4373
    Illegal EcoRI site found at 5489
    Illegal EcoRI site found at 5573
    Illegal EcoRI site found at 8628
    Illegal BglII site found at 1115
    Illegal BglII site found at 3155
    Illegal BglII site found at 4519
    Illegal BamHI site found at 3253
    Illegal BamHI site found at 3268
    Illegal BamHI site found at 6967
    Illegal BamHI site found at 7425
    Illegal BamHI site found at 8398
    Illegal XhoI site found at 337
    Illegal XhoI site found at 1945
    Illegal XhoI site found at 2590
    Illegal XhoI site found at 3064
    Illegal XhoI site found at 5594
    Illegal XhoI site found at 6261
    Illegal XhoI site found at 6394
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 2173
    Illegal EcoRI site found at 2614
    Illegal EcoRI site found at 4373
    Illegal EcoRI site found at 5489
    Illegal EcoRI site found at 5573
    Illegal EcoRI site found at 8628
    Illegal PstI site found at 2758
    Illegal PstI site found at 2857
    Illegal PstI site found at 3442
    Illegal PstI site found at 4400
    Illegal PstI site found at 4628
    Illegal PstI site found at 6138
    Illegal PstI site found at 7152
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 2173
    Illegal EcoRI site found at 2614
    Illegal EcoRI site found at 4373
    Illegal EcoRI site found at 5489
    Illegal EcoRI site found at 5573
    Illegal EcoRI site found at 8628
    Illegal PstI site found at 2758
    Illegal PstI site found at 2857
    Illegal PstI site found at 3442
    Illegal PstI site found at 4400
    Illegal PstI site found at 4628
    Illegal PstI site found at 6138
    Illegal PstI site found at 7152
    Illegal NgoMIV site found at 2701
    Illegal NgoMIV site found at 4090
    Illegal NgoMIV site found at 4264
    Illegal NgoMIV site found at 4414
    Illegal NgoMIV site found at 7588
    Illegal AgeI site found at 4715
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The part is a MoClo Lv.1 part, assembled in in the acceptor Lv1/2 entry vector whose ori is “BBa_K2560036”, and it's resistance cassette is “BBa_K3726039” (MARK_Lv0_AmpR). This part has been assembled following the marburg collection standard, then the transcriptional unit is flanked by the parts “BBa_K3726110” 5CON1(H)_nNS4-up (PCC 11801) and “BBa_K3726111” 3CON5(H)_nNS4-down (PCC 11801) allowing the Integration within the PCC 11801 Genome.

Source

This construct has been made by golden gate reaction.

References

H. Yu, X. Li, F. Duchoud, D. Chuang and J. Liao, "Augmenting the Calvin–Benson–Bassham cycle by a synthetic malyl-CoA-glycerate carbon fixation pathway", 2021