Composite

Part:BBa_K3726053:Design

Designed by: Santiago Barrag�n Ariza   Group: iGEM21_MADRID_UCM   (2021-10-19)


Lv.1_PKMCG-A


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 9525
    Illegal EcoRI site found at 1128
    Illegal SpeI site found at 10488
    Illegal PstI site found at 575
    Illegal PstI site found at 839
    Illegal PstI site found at 4411
    Illegal PstI site found at 5040
    Illegal PstI site found at 5537
    Illegal PstI site found at 6136
    Illegal PstI site found at 7074
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1128
    Illegal EcoRI site found at 9525
    Illegal NheI site found at 9177
    Illegal NheI site found at 9204
    Illegal SpeI site found at 10488
    Illegal PstI site found at 575
    Illegal PstI site found at 839
    Illegal PstI site found at 4411
    Illegal PstI site found at 5040
    Illegal PstI site found at 5537
    Illegal PstI site found at 6136
    Illegal PstI site found at 7074
    Illegal NotI site found at 9531
    Illegal NotI site found at 10495
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1128
    Illegal EcoRI site found at 9525
    Illegal BamHI site found at 505
    Illegal BamHI site found at 2950
    Illegal BamHI site found at 10070
    Illegal XhoI site found at 9401
    Illegal XhoI site found at 11519
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 9525
    Illegal suffix found in sequence at 10488
    Illegal EcoRI site found at 1128
    Illegal PstI site found at 575
    Illegal PstI site found at 839
    Illegal PstI site found at 4411
    Illegal PstI site found at 5040
    Illegal PstI site found at 5537
    Illegal PstI site found at 6136
    Illegal PstI site found at 7074
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 9525
    Illegal EcoRI site found at 1128
    Illegal XbaI site found at 9540
    Illegal SpeI site found at 10488
    Illegal PstI site found at 575
    Illegal PstI site found at 839
    Illegal PstI site found at 4411
    Illegal PstI site found at 5040
    Illegal PstI site found at 5537
    Illegal PstI site found at 6136
    Illegal PstI site found at 7074
    Illegal NgoMIV site found at 2527
    Illegal NgoMIV site found at 4636
    Illegal NgoMIV site found at 5096
    Illegal NgoMIV site found at 8992
    Illegal NgoMIV site found at 9134
    Illegal AgeI site found at 6649
    Illegal AgeI site found at 6732
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 9555
    Illegal BsaI.rc site found at 10472


Design Notes

The part is a MoClo Lv.1 part, assembled in in the acceptor Lv1/2 entry vector whose ori is “BBa_K2560036”, and it's resistance cassette is “BBa_K3726039” (MARK_Lv0_AmpR). This part has been assembled following the marburg collection standard, then the transcriptional unit is flanked by the parts “BBa_K3726112” 5CON1(H)_nNS5-up (PCC 11801) and “BBa_K3726113” 3CON5(H)_nNS5-down (PCC 11801) allowing the Integration within the PCC 11801 Genome.

Source

This construct has been made by golden gate reaction.

References

H. Yu, X. Li, F. Duchoud, D. Chuang and J. Liao, "Augmenting the Calvin–Benson–Bassham cycle by a synthetic malyl-CoA-glycerate carbon fixation pathway", 2021.