Part:BBa_K3726051
L1_BOH1C-FRRRF
This is a composite part that corresponds with a level 1 MoClo part. It is a transcriptional unit for the expression of the part “BBa_K3726018” CDS_Lv0_BOH_1_C_FRRRF , the last two enzymes of synthetic butanol biosynthesis operon linked with a flexible and rigid tandem without including a stop codon between themselves.
The part in its upstream region, has the connector 5'Con3 “K2560067” that will allow the assembly of a Lv2 construct following the marburg collection standard. In its downstream region has a homology region “BBa_K3726105” 3CON5(H)_NS1(mod)-down (PCC 11801) for homologous recombination within the genome of PCC 11801.
Likewise, a Kanamycin resistance cassette is included downstream the transcriptional unit region.
Expression is controlled by the medium-strong synthetic constitutive promoter J23119 “BBa_K2560031”and by the strong synthetic RBS, RBS_PduP “BBa_K3726090”.
Sequence and Features
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 1751
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 431
Illegal NheI site found at 454
Illegal NheI site found at 757
Illegal NheI site found at 985
Illegal PstI site found at 1751 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 2146
Illegal BglII site found at 2263
Illegal BglII site found at 4682
Illegal XhoI site found at 3366
Illegal XhoI site found at 4209 - 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 1751
- 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 1751
- 1000COMPATIBLE WITH RFC[1000]
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