Composite

Part:BBa_K3726050

Designed by: Santiago Barrag�n Ariza   Group: iGEM21_MADRID_UCM   (2021-10-19)


BOH3_LV1 (B)

This is a composite part that corresponds with a level 1 MoClo part. It is a transcriptional unit for the expression of the part BBa_K3726011 CDS_Lv0_BOH_3 , a butanol biosynthesis operon.

The part is flanked by two homology regions “BBa_K3726104” 5CON1(H)_NS1(mod)-up (PCC 11801) and “BBa_K3726105 ” 3CON5(H)_NS1(mod)-down (PCC 11801) for the double homologous recombination within the genome of PCC 11801.

Likewise, a Kanamycin resistance cassette is included downstream the transcriptional unit region.

Expression is controlled by the medium-strong synthetic constitutive promoter J23119 “BBa_K2560031” and the strong synthetic RBS, RBS_NphT7 “BBa_K3726088”


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1533
    Illegal PstI site found at 18
    Illegal PstI site found at 1438
    Illegal PstI site found at 1686
    Illegal PstI site found at 2987
    Illegal PstI site found at 6002
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1533
    Illegal NheI site found at 1003
    Illegal NheI site found at 1026
    Illegal NheI site found at 5008
    Illegal NheI site found at 5236
    Illegal PstI site found at 18
    Illegal PstI site found at 1438
    Illegal PstI site found at 1686
    Illegal PstI site found at 2987
    Illegal PstI site found at 6002
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1533
    Illegal BglII site found at 6347
    Illegal BglII site found at 6464
    Illegal BglII site found at 8883
    Illegal BamHI site found at 3070
    Illegal BamHI site found at 3409
    Illegal XhoI site found at 4342
    Illegal XhoI site found at 7567
    Illegal XhoI site found at 8410
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1533
    Illegal PstI site found at 18
    Illegal PstI site found at 1438
    Illegal PstI site found at 1686
    Illegal PstI site found at 2987
    Illegal PstI site found at 6002
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1533
    Illegal PstI site found at 18
    Illegal PstI site found at 1438
    Illegal PstI site found at 1686
    Illegal PstI site found at 2987
    Illegal PstI site found at 6002
    Illegal NgoMIV site found at 1177
    Illegal NgoMIV site found at 1277
    Illegal NgoMIV site found at 1583
    Illegal NgoMIV site found at 3665
    Illegal AgeI site found at 594
    Illegal AgeI site found at 1955
    Illegal AgeI site found at 3193
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
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