Composite

Part:BBa_K3726049

Designed by: Santiago Barrag�n Ariza   Group: iGEM21_MADRID_UCM   (2021-10-19)

BOH3_LV1 (A)

This is a composite part that corresponds with a level 1 MoClo part. It is a transcriptional unit for the expression of the part BBa_K3726011 CDS_Lv0_BOH_3 a butanol biosynthesis operon.

The part is flanked by two homology regions “BBa_K3726104” 5CON1(H)_NS1(mod)-up (PCC 11801) and “BBa_K3726105 ” 3CON5(H)_NS1(mod)-down (PCC 11801) for the double homologous recombination within the genome of PCC 11801.

Likewise, a Kanamycin resistance cassette is included downstream the transcriptional unit region.

Expression is controlled by the strong constitutive promoter Prbcl-m36 “BBa_K3726080 ” and the strong synthetic RBS, RBS_NphT7 “BBa_K3726088”

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1786
    Illegal PstI site found at 18
    Illegal PstI site found at 1221
    Illegal PstI site found at 1691
    Illegal PstI site found at 1939
    Illegal PstI site found at 3240
    Illegal PstI site found at 6255
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1786
    Illegal NheI site found at 5261
    Illegal NheI site found at 5489
    Illegal PstI site found at 18
    Illegal PstI site found at 1221
    Illegal PstI site found at 1691
    Illegal PstI site found at 1939
    Illegal PstI site found at 3240
    Illegal PstI site found at 6255
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1786
    Illegal BglII site found at 6600
    Illegal BglII site found at 6717
    Illegal BglII site found at 9136
    Illegal BamHI site found at 3323
    Illegal BamHI site found at 3662
    Illegal XhoI site found at 4595
    Illegal XhoI site found at 7820
    Illegal XhoI site found at 8663
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1786
    Illegal PstI site found at 18
    Illegal PstI site found at 1221
    Illegal PstI site found at 1691
    Illegal PstI site found at 1939
    Illegal PstI site found at 3240
    Illegal PstI site found at 6255
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1786
    Illegal PstI site found at 18
    Illegal PstI site found at 1221
    Illegal PstI site found at 1691
    Illegal PstI site found at 1939
    Illegal PstI site found at 3240
    Illegal PstI site found at 6255
    Illegal NgoMIV site found at 1430
    Illegal NgoMIV site found at 1530
    Illegal NgoMIV site found at 1836
    Illegal NgoMIV site found at 3918
    Illegal AgeI site found at 594
    Illegal AgeI site found at 2208
    Illegal AgeI site found at 3446
  • 1000
    COMPATIBLE WITH RFC[1000]
[edit]
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Parameters
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