Composite

Part:BBa_K3726047:Design

Designed by: Santiago Barrag�n Ariza   Group: iGEM21_MADRID_UCM   (2021-10-19)

BOH2_A_LV1


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1230
    Illegal EcoRI site found at 1873
    Illegal PstI site found at 18
    Illegal PstI site found at 1778
    Illegal PstI site found at 2026
    Illegal PstI site found at 3327
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1230
    Illegal EcoRI site found at 1873
    Illegal PstI site found at 18
    Illegal PstI site found at 1778
    Illegal PstI site found at 2026
    Illegal PstI site found at 3327
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1230
    Illegal EcoRI site found at 1873
    Illegal BamHI site found at 3410
    Illegal BamHI site found at 3749
    Illegal XhoI site found at 4682
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1230
    Illegal EcoRI site found at 1873
    Illegal PstI site found at 18
    Illegal PstI site found at 1778
    Illegal PstI site found at 2026
    Illegal PstI site found at 3327
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1230
    Illegal EcoRI site found at 1873
    Illegal PstI site found at 18
    Illegal PstI site found at 1778
    Illegal PstI site found at 2026
    Illegal PstI site found at 3327
    Illegal NgoMIV site found at 1517
    Illegal NgoMIV site found at 1617
    Illegal NgoMIV site found at 1923
    Illegal NgoMIV site found at 4005
    Illegal AgeI site found at 594
    Illegal AgeI site found at 2295
    Illegal AgeI site found at 3533
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The part is a MoClo Lv.1 part, assembled in in the acceptor Lv1 entry vector whose ori is “BBa_K2560036”, and it's resistance cassette is “BBa_K3726039” (MARK_Lv0_AmpR). This part has been assembled following the marburg collection standard, then the transcriptional unit is flanked by the part “BBa_K3726104” (5CON1(H)_NS1(mod)-up (PCC 11801)) that allows homologous recombination within the PCC 11801 Genome, and by the part 3'Con2 “BBa_K2560071” that will allow the assembly of a Lv2 construct.

Source

This construct has been made by golden gate reaction.

References

X. Liu, R. Miao, P. Lindberg and P. Lindblad, "Modular engineering for efficient photosynthetic biosynthesis of 1-butanol from CO2in cyanobacteria", 2021.