Composite

Part:BBa_K3726047

Designed by: Santiago Barrag�n Ariza   Group: iGEM21_MADRID_UCM   (2021-10-19)

BOH2_A_LV1

This is a composite part that corresponds with a level 1 MoClo part. It is a transcriptional unit for the expression of the part “BBa_K3726015” CDS_Lv0_BOH_2_A , the first four enzymes of a synthetic butanol biosynthesis operon.

The part in its upstream region has a homology region “BBa_K3726104” 5CON1(H)_NS1(mod)-up (PCC 11801) for homologous recombination within the genome of PCC 11801. In the downstream region, the part has the connector 3'Con2 “BBa_K2560071” that will allow the assembly of a Lv2 construct following the marburg collection standard.

Expression is controlled by the strong constitutive promoter P_PpsbA*-RiboJ “BBa_K3726079” and the strong synthetic RBS, RBS_NphT7 “BBa_K3726088”.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1230
    Illegal EcoRI site found at 1873
    Illegal PstI site found at 18
    Illegal PstI site found at 1778
    Illegal PstI site found at 2026
    Illegal PstI site found at 3327
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1230
    Illegal EcoRI site found at 1873
    Illegal PstI site found at 18
    Illegal PstI site found at 1778
    Illegal PstI site found at 2026
    Illegal PstI site found at 3327
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1230
    Illegal EcoRI site found at 1873
    Illegal BamHI site found at 3410
    Illegal BamHI site found at 3749
    Illegal XhoI site found at 4682
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1230
    Illegal EcoRI site found at 1873
    Illegal PstI site found at 18
    Illegal PstI site found at 1778
    Illegal PstI site found at 2026
    Illegal PstI site found at 3327
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1230
    Illegal EcoRI site found at 1873
    Illegal PstI site found at 18
    Illegal PstI site found at 1778
    Illegal PstI site found at 2026
    Illegal PstI site found at 3327
    Illegal NgoMIV site found at 1517
    Illegal NgoMIV site found at 1617
    Illegal NgoMIV site found at 1923
    Illegal NgoMIV site found at 4005
    Illegal AgeI site found at 594
    Illegal AgeI site found at 2295
    Illegal AgeI site found at 3533
  • 1000
    COMPATIBLE WITH RFC[1000]
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Categories
Parameters
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