Part:BBa_K3726046:Design

BOH1_C_Lv1

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal PstI site found at 1723
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 431
  Illegal NheI site found at 454
  Illegal NheI site found at 729
  Illegal NheI site found at 957
  Illegal PstI site found at 1723
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 2057
  Illegal BglII site found at 2174
  Illegal BglII site found at 4593
  Illegal XhoI site found at 3277
  Illegal XhoI site found at 4120
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal PstI site found at 1723
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal PstI site found at 1723
  
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

The part is a MoClo Lv.1 part, assembled in in the acceptor Lv1 entry vector whose ori is “BBa_K2560036”, and it's resistance cassette is “BBa_K3726039” (MARK_Lv0_AmpR). This part has been assembled following the marburg collection standard, then the transcriptional unit is flanked by the part 5'Con3 “BBa_K2560067” in its upstream region will allow the assembly of a Lv2 construct following the marburg collection standard. And by the part “BBa_K3726105” 3CON5(H)_NS1(mod)-down (PCC 11801) in its downstream region, which is a homology region for homologous recombination within the genome of PCC 11801.

Source

This construct has been made by golden gate reaction.

References

X. Liu, R. Miao, P. Lindberg and P. Lindblad, "Modular engineering for efficient photosynthetic biosynthesis of 1-butanol from CO2in cyanobacteria", 2021.