Composite

Part:BBa_K3726045:Design

Designed by: Santiago Barrag�n Ariza   Group: iGEM21_MADRID_UCM   (2021-10-19)

BOH1_B_LV1


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 654
    Illegal PstI site found at 903
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 654
    Illegal PstI site found at 903
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 986
    Illegal BamHI site found at 1325
    Illegal XhoI site found at 2234
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 654
    Illegal PstI site found at 903
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 654
    Illegal PstI site found at 903
    Illegal NgoMIV site found at 1581
    Illegal AgeI site found at 109
    Illegal AgeI site found at 1109
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The part is a MoClo Lv.1 part, assembled in in the acceptor Lv1 entry vector whose ori is “BBa_K2560036”, and it's resistance cassette is “BBa_K3726039” (MARK_Lv0_AmpR). This part has been assembled following the marburg collection standard, then the transcriptional unit is flanked by two connectors that will allow the assembly of a Lv2 construct. In its upstream region the connector is 5'Con2 BBa_K2560066, and in its downstream region 3'Con2 BBa_K2560071.

Source

This construct has been made by golden gate reaction.

References

X. Liu, R. Miao, P. Lindberg and P. Lindblad, "Modular engineering for efficient photosynthetic biosynthesis of 1-butanol from CO2in cyanobacteria", 2021.