Composite

Part:BBa_K3726045

Designed by: Santiago Barrag�n Ariza   Group: iGEM21_MADRID_UCM   (2021-10-19)

BOH1_B_LV1

This is a composite part that corresponds with a level 1 MoClo part. It is a transcriptional unit for the expression of the part “BBa_K3726013” CDS_Lv0_BOH_1_B , the third and the fourth two enzymes of synthetic butanol biosynthesis operon.

The part is flanked by two connectors that will allow the assembly of a Lv2 construct. In its upstream region the connector is 5'Con2 K2560066, and in its downstream region 3'Con2 K2560071 .

Expression is controlled by the strong constitutive promoter Prbcl-m36 “BBa_K3726080 ”and the strong synthetic RBS , RBS_BCD2(mod) “BBa_K3726086”.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 654
    Illegal PstI site found at 903
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 654
    Illegal PstI site found at 903
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 986
    Illegal BamHI site found at 1325
    Illegal XhoI site found at 2234
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 654
    Illegal PstI site found at 903
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 654
    Illegal PstI site found at 903
    Illegal NgoMIV site found at 1581
    Illegal AgeI site found at 109
    Illegal AgeI site found at 1109
  • 1000
    COMPATIBLE WITH RFC[1000]
[edit]
Categories
Parameters
None