Coding

Part:BBa_K3726037:Design

Designed by: Santiago Barrag�n Ariza   Group: iGEM21_MADRID_UCM   (2021-10-06)


CDS_mcl


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 839
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 839
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 839
    Illegal BamHI site found at 609
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 839
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 839
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part corresponds with the codon optimized coding sequence of mcl enzyme. Codon optimization has been performed using DeNovo DNA Software, adjusting the codon usage for high expression in Synechococcus elongatus PCC7942. In addition the part sequence has been optimized to improve mRNA stability removing internal recognition sites for endonucleases

Source

Coding sequence of this Malyl-CoA lyase has been found within the genome of Rhodobacter sphaeroides. Uniprot reference: https://www.uniprot.org/uniprot/Q3J5L6


References

H. Yu, X. Li, F. Duchoud, D. Chuang and J. Liao, "Augmenting the Calvin–Benson–Bassham cycle by a synthetic malyl-CoA-glycerate carbon fixation pathway", 2021.