Coding

Part:BBa_K3726035:Design

Designed by: Santiago Barrag�n Ariza   Group: iGEM21_MADRID_UCM   (2021-10-06)


CDS_mtkB


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 279
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 279
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 94
    Illegal BamHI site found at 552
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 279
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 279
    Illegal NgoMIV site found at 715
  • 1000
    COMPATIBLE WITH RFC[1000]

Design Notes

This part has been designed to be assembled within the part "BBa_K3726038" thought Gibson.

Source

Coding sequence of Malate--CoA ligase subunit alpha has been found within the genome of Methylococcus Capsulatus. Uniprot reference:https://www.uniprot.org/uniprot/P53595

References

H. Yu, X. Li, F. Duchoud, D. Chuang and J. Liao, "Augmenting the Calvin–Benson–Bassham cycle by a synthetic malyl-CoA-glycerate carbon fixation pathway", 2021.