Coding

Part:BBa_K3726035

Designed by: Santiago Barragán Ariza   Group: iGEM21_MADRID_UCM   (2021-10-06)


CDS_mtkB

This part corresponds with the protein coding sequence for an Malate--CoA ligase subunit alpha.

The codified enzyme from Methylococcus Capsulatus catalyzes the ATP-mediated condensation of CoA with malate, forming Malyl-CoA. The enzyme has two subunits alpha (CDS mtkA) and beta (CDS mtkB).


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 279
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 279
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 94
    Illegal BamHI site found at 552
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 279
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 279
    Illegal NgoMIV site found at 715
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None