Coding

Part:BBa_K3726033

Designed by: Santiago Barragán Ariza   Group: iGEM21_MADRID_UCM   (2021-10-06)


CDS_mtkA

This part corresponds with the protein coding sequence for an Malate CoA ligase subunit beta.

The codified enzyme from Methylococcus Capsulatus catalyzes the ATP-mediated condensation of CoA with malate, forming Malyl-CoA. The enzyme has two subunits alpha (CDS mtkA) and beta (CDS mtkB).

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 463
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 37
    Illegal PstI site found at 463
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 586
    Illegal XhoI site found at 719
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 463
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 463
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None