Coding

Part:BBa_K3726031

Designed by: Santiago Barragán Ariza   Group: iGEM21_MADRID_UCM   (2021-10-06)


gcl_CDS

This part corresponds with the protein coding sequence for a Glyoxylate carboligase.

The codified enzyme from Cupriavidus necator catalyzes the decarboxylation of glyoxylate to tartronate semialdehyde.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 514
    Illegal EcoRI site found at 1630
    Illegal EcoRI site found at 1714
    Illegal PstI site found at 541
    Illegal PstI site found at 769
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 514
    Illegal EcoRI site found at 1630
    Illegal EcoRI site found at 1714
    Illegal PstI site found at 541
    Illegal PstI site found at 769
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 514
    Illegal EcoRI site found at 1630
    Illegal EcoRI site found at 1714
    Illegal BglII site found at 660
    Illegal XhoI site found at 1735
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 514
    Illegal EcoRI site found at 1630
    Illegal EcoRI site found at 1714
    Illegal PstI site found at 541
    Illegal PstI site found at 769
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 514
    Illegal EcoRI site found at 1630
    Illegal EcoRI site found at 1714
    Illegal PstI site found at 541
    Illegal PstI site found at 769
    Illegal NgoMIV site found at 231
    Illegal NgoMIV site found at 405
    Illegal NgoMIV site found at 555
    Illegal AgeI site found at 856
  • 1000
    COMPATIBLE WITH RFC[1000]


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Parameters
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