Coding

Part:BBa_K3726023:Design

Designed by: Santiago Barragán Ariza   Group: iGEM21_MADRID_UCM   (2021-10-05)


CDS_mdh (E. Coli)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part has been designed to be assembled within the part "BBa_K3726028" through Gibson.

Source

Coding sequence of this Malate dehydrogenase has been obtained from Escherichia coli (strain BL21) genome thought PCR. Uniprot reference: https://www.uniprot.org/uniprot/A0A140N775


References

"Regulation of malate dehydrogenase (mdh) gene expression in Escherichia coli in response to oxygen, carbon, and heme availability | Journal of Bacteriology", Journal of Bacteriology, 2021. [Online]. Available: https://doi.org/10.1128/jb.177.22.6652-6656.1995.