Coding

Part:BBa_K3726004:Design

Designed by: Santiago Barragán Ariza   Group: iGEM21_MADRID_UCM   (2021-10-04)


CDS_PduP


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 1246
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 252
    Illegal NheI site found at 480
    Illegal PstI site found at 1246
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 1246
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 1246
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part corresponds with the codon optimized coding sequence of PduP enzyme. Codon optimization has been performed using DeNovo DNA Software, adjusting the codon usage for high expression in Synechococcus elongatus PCC7942. In addition the part sequence has been optimized to improve mRNA stability removing internal recognition sites for endonucleases

Source

Coding sequence of this Aldehyde dehydrogenase has been found within the genome of Salmonella enterica. Uniprot reference: https://www.uniprot.org/uniprot/A0A3V3TWW8

References

C. Fan et al., "Short N-terminal sequences package proteins into bacterial microcompartments", 2021.

C. Fan, S. Cheng, S. Sinha and T. Bobik, "Interactions between the termini of lumen enzymes and shell proteins mediate enzyme encapsulation into bacterial microcompartments", 2021.