Coding

Part:BBa_K3726002

Designed by: Santiago Barragán Ariza   Group: iGEM21_MADRID_UCM   (2021-10-04)


CDS_Ccr

This part corresponds with the protein coding sequence for an Crotonyl-CoA reductase

The codified enzyme from Streptomyces Collinus catalyzes the reversible oxidation step of crotonyl-coA to Butyril-coA. NADPH-dependent enzyme. Its optimum temperature is around 40 degrees.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 85
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 85
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 168
    Illegal BamHI site found at 507
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 85
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 85
    Illegal NgoMIV site found at 763
    Illegal AgeI site found at 291
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None