Intermediate

Part:BBa_K3725082

Designed by: Alice Hou, Michelle Jing, Monica Cho, Janet Standeven, Megan McSweeney   Group: iGEM21_Lambert_GA   (2021-10-14)


Improved Fusarium Toehold Switch Sequence
The Improved Fusarium Toehold Switch Sequence was derived from NUPACK and was constructed with the second lowest normalized ensemble defect rate possible. The sequence forms a hairpin loop, hiding machinery necessary for translation. However, in the presence of the FRP1 gene of Fusarium oxysporum, a plant pathogen, the hairpin loop unravels, allowing for expression of the downstream gene to occur. As a result, this sequence can be paired with a reporter gene to function as a biosensor for the presence of the plant disease F. oxysporum.



Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Parameters
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