Coding

Part:BBa_K3722006:Design

Designed by: Yongzhu Li   Group: iGEM21_NWU-CHINA-A   (2021-09-29)


Fre-Linker-SttH


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 961
    Illegal NgoMIV site found at 1219
    Illegal AgeI site found at 1448
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 424


Design Notes

Fre was effective as a soluble N-terminal tag for SttH.



Source

Assembly

References